Focused Gut DNA Analysis Tool

This profile provides a focused gut microbiome DNA analysis tool that identifies 45 targeted analytes across six phyla using a CE-marked multiplex PCR system providing dysbiosis, abundance and diversity assessments of the gut microbiome based on these 45 peer reviewed analytes shown to contribute to dysbiosis and other chronic disease states.

What Patients Might Benefit from GI360™ Profile

Gastrointestinal stool analysis is indicated for all chronic GI problems, and many systemic diseases.

• Allergies

• Anxiety

• Atopic Dermatitis

• Arthritis / Joint Pain

• Autoimmune Disorders

• Bowel Pattern Changes

• Cardiovascular Disease

• Celiac Disease

• Cognition Issues

• Crohn’s Disease

• Depression

• Diabetes

• Diarrhea, Constipation, Abdominal Distension

• Diverticulosis / Diverticulitis

• Eczema

• Food Sensitivities

• IBD / IBS

• Early Satiety

• Gas / Bloating / Abdominal Pain

• Gastrointestinal Symptoms

• Indigestion

• MCASYd

• Metabolic Syndrome (HTN, obesity, T2DM, lipid abnormalities)

• Migraines

• Mood Disorders

• Mucosal Barrier Dysfunction

• Psoriasis

• Rashes

• Skin Issues

The GI360™ Microbiome Profile provides a focused gut microbiome DNA analysis.

• PCR Analysis for the Dysbiosis, Abundance, and Diversity of Key Bacterial Populations of the GI Microbiome

Microbiome Abundance and Diversity – The GI360 Microbiome Profile is a gut microbiota DNA analysis tool that identifies and characterizes the abundance and diversity of more than 45 targeted analytes that peer-reviewed research has shown to contribute to dysbiosis and other chronic disease states.

The Dysbiosis and Diversity Index These indexes are calculated from the results of the Microbiome Profile, with scores ranging from 1 to 5, and do not include consideration of dysbiotic and pathogenic bacteria, yeast, parasites and viruses that may be reported in subsequent sections of the GI360 test. A dysbiosis score above 2 indicates dysbiosis; a microbiota profile that differs from the defined normobiotic reference population.  The higher the score above 2, the more the sample deviates from the normobiotic profile. A diversity score of 3 indicates an expected amount of diversity, with 4 & 5 indicating an increased distribution of bacteria based on the number of different species and their abundance in the sample, calculated based on Shannon’s diversity index. Scores of 1 or 2 indicate less diversity than the defined normobiotic reference population.

Sample Report - GI360 Microbiome

What Patients Might Benefit from GI360™ Profile

Gastrointestinal stool analysis is indicated for all chronic GI problems, and many systemic diseases.

• Allergies

• Anxiety

• Atopic Dermatitis

• Arthritis / Joint Pain

• Autoimmune Disorders

• Bowel Pattern Changes

• Cardiovascular Disease

• Celiac Disease

• Cognition Issues

• Crohn’s Disease

• Depression

• Diabetes

• Diarrhea, Constipation, Abdominal Distension

• Diverticulosis / Diverticulitis

• Eczema

• Food Sensitivities

• IBD / IBS

• Early Satiety

• Gas / Bloating / Abdominal Pain

• Gastrointestinal Symptoms

• Indigestion

• MCASYd

• Metabolic Syndrome (HTN, obesity, T2DM, lipid abnormalities)

• Migraines

• Mood Disorders

• Mucosal Barrier Dysfunction

• Psoriasis

• Rashes

• Skin Issues

The GI360™ Essentials Profile offers an extensive assessment of the gastrointestinal microbiome.

• PCR Analysis for the Abundance and Diversity of Key Bacterial Populations of the GI Microbiome

• PCR Detection of Pathogenic Bacteria, Viruses, and Parasites

• Comprehensive Parasitology by Microscopy

• MALDI-TOF ID of Cultured Bacteria and Yeast

• Standardized Susceptibility Testing of Isolated Bacteria and Yeast

GI360 Essentials Stool Profile: an innovative, comprehensive, and clinically applicable stool profile, utilizing multiplex PCR molecular technology coupled with growth-based culture and ID by MALDI-TOF, sensitive biochemical assays, and microscopy to detect and assess the status of pathogens, viruses, parasites, and bacteria that may be contributing to acute or chronic gastrointestinal symptoms and disease.

Microbiome Abundance and Diversity – The GI360 Essentials Profile includes a gut microbiota DNA analysis tool that identifies and characterizes the abundance and diversity of more than 45 targeted analytes that peer-reviewed research has shown to contribute to dysbiosis and other chronic disease states.

The GI360 Essentials Profile can identify the presence of pathogenic viruses, bacteria, and parasites using multiplexed, real-time PCR. Viruses are the primary cause of acute diarrhea, and the least commonly tested. The identification of pathogenic bacteria, viruses and parasites improves treatment strategies and patient outcomes.

The Dysbiosis and Diversity Index These indexes are calculated from the results, with scores ranging from 1 to 5, and do not include consideration of dysbiotic and pathogenic bacteria, yeast, parasites, and viruses that may be reported in subsequent sections of the GI360 Essentials Profile. A dysbiosis score above 2 indicates dysbiosis; a microbiota profile that differs from the defined normobiotic reference population.  The higher the score above 2, the more the sample deviates from the normobiotic profile. A diversity score of 3 indicates an expected amount of diversity, with 4 & 5 indicating an increased distribution of bacteria based on the number of different species and their abundance in the sample, calculated based on Shannon’s diversity index. Scores of 1 or 2 indicate less diversity than the defined normobiotic reference population. This expanded view of clinically significant bacteria offers actionable data to the practitioner, particularly in combination with the complementary methodologies employed in the GI360 Essentials Profile.

Sample Report - GI360 Essentials

hat Patients Might Benefit from GI360™ Profile

Gastrointestinal stool analysis is indicated for all chronic GI problems, and many systemic diseases.

• Allergies

• Anxiety

• Atopic Dermatitis

• Arthritis / Joint Pain

• Autoimmune Disorders

• Bowel Pattern Changes

• Cardiovascular Disease

• Celiac Disease

• Cognition Issues

• Crohn’s Disease

• Depression

• Diabetes

• Diarrhea, Constipation, Abdominal Distension

• Diverticulosis / Diverticulitis

• Eczema

• Food Sensitivities

• IBD / IBS

• Early Satiety

• Gas / Bloating / Abdominal Pain

• Gastrointestinal Symptoms

• Indigestion

• MCASYd

• Metabolic Syndrome (HTN, obesity, T2DM, lipid abnormalities)

• Migraines

• Mood Disorders

• Mucosal Barrier Dysfunction

• Psoriasis

• Rashes

• Skin Issues

The GI360™ Stool Profile offers extensive assessment of the gastrointestinal microbiome.

• PCR Analysis for the Abundance and Diversity ofKey Bacterial Populations of the GI Microbiome

• PCR Detection of Pathogenic Bacteria, Viruses, and Parasites

• Comprehensive Parasitology by Microscopy

• MALDI-TOF ID of Cultured Bacteria and Yeast

• Broad Range of Stool Chemistry Markers

• Standardized Susceptibility Testing of Isolated Bacteria and Yeast

GI360 Profile: an innovative, comprehensive, and clinically applicable stool profile, utilizing multiplex PCR molecular technology coupled with growth-based culture and ID by MALDI-TOF, sensitive biochemical assays and microscopy to detect and assess the status of pathogens, viruses, parasites and bacteria that may be contributing to acute or chronic gastrointestinal symptoms and disease.

Microbiome Abundance and Diversity – The GI360 Profile is a gut microbiota DNA analysis tool that identifies and characterizes the abundance and diversity of more than 45 targeted analytes that peer-reviewed research has shown to contribute to dysbiosis and other chronic disease states.

The GI360 Profile can identify the presence of pathogenic viruses, bacteria, and parasites using multiplexed, real-time PCR. Viruses are the primary cause of acute diarrhea, and the least commonly tested. The identification of pathogenic bacteria, viruses, and parasites improves treatment strategies and patient outcomes.

The Dysbiosis and Diversity Index These indexes are calculated from the results of the Microbiome Profile, with scores ranging from 1 to 5, and do not include consideration of dysbiotic and pathogenic bacteria, yeast, parasites and viruses that may be reported in subsequent sections of the GI360 Profile. A dysbiosis score above 2 indicates dysbiosis; a microbiota profile that differs from the defined normobiotic reference population.  The higher the score above 2, the more the sample deviates from the normobiotic profile. A diversity score of 3 indicates an expected amount of diversity, with 4 & 5 indicating an increased distribution of bacteria based on the number of different species and their abundance in the sample, calculated based on Shannon’s diversity index. Scores of 1 or 2 indicate less diversity than the defined normobiotic reference population. This expanded view of clinically significant bacteria offers actionable data to the practitioner, particularly in combination with the complementary methodologies employed in the GI360™ Profile.

Sample Report - GI360 Profile

We recommend GI360 as they have the GI microbiome included!

Imbalances in gastrointestinal function can lead to both local and systemic health issues, with the gut microbiome playing a vital role in supporting overall health.

Our Comprehensive Stool Analysisprofile helps identify the underlying causes of acute and chronic symptoms, enabling providers to create personalized treatment plans.

The Comprehensive Stool Analysis measures key markers of digestion, absorption, and intestinal function, including a comprehensive bacteriology and yeast culture to detect beneficial, imbalanced, and dysbiotic flora. We use gold standard methods, such as PCR, to identify infectious pathogens and parasites, and perform antimicrobial susceptibility testing on cultured bacterial and fungal species to recommend prescriptive and natural agents.

What Patients Might Benefit from the Comprehensive Stool Analysis

The Comprehensive Stool Analysis is indicated for all chronic GI problems, for acute bowel pattern changes, and for many systemic diseases.

• Allergies

• Autoimmune Disorders

• Cardiovascular Disease

• Celiac Disease

• Diabetes

• Diarrhea, Constipation, Abdominal Distension

• Food Sensitivities

• Gastrointestinal Symptoms

• IBD

• IBS

• Immune System Imbalances

• Inflammation

• Joint Pain

• Leaky Gut

• Malnutrition

• Mood Disorders

• Neurological Disorders

• Weight Concerns

Why Conduct a Comprehensive Stool Analysis?

The Comprehensive Stool Analysis + Parasitology (CSAP) is an essential diagnostic tool that enables practitioners to assess the status of beneficial and imbalanced commensal bacteria, pathogenic bacteria, yeast/fungus, and parasites. The identification of pathogenic species and susceptibility testing assists in the selection of the most suitable pharmaceutical or natural treatment agents.

Our Comprehensive Stool Analysis test measures:

• Digestion and absorption of nutrients (Pancreatic Elastase, Muscle Fibers, and Vegetable Fibers)

• Elimination efficiency of undigested food residues and toxins

• Assessment of the gut microbiome, measuring levels of healthy bacterial flora versus potentially pathogenic bacteria species, yeast, and parasites

• Culture and sensitivities of pathogenic yeast and bacteria

• Infectious pathogens (EIA evaluation for Campylobacter, Enterohemorrhagic E. coli cytotoxin, Giardia lamblia, and Cryptosporidium)

• Indices and markers of intestinal immune function (Secretory IgA)

• Indices and markers of inflammation (Calprotectin, Lactoferrin, and Lysozyme)

• Indices and markers of intestinal physiology and of intestinal health (Presence of RBC, WBC, Mucus, Occult Blood, Fecal pH, and Short Chain Fatty Acids)

Efficient remediation of GI dysfunctions involves a comprehensive approach that includes elimination of pathogens and irritants, supplementation of hydrochloric acid, pancreatic enzymes, and pre- and probiotics, and repair of the mucosal barrier.

Sample Report - Comprehensive Stool Analysis

IThe Microbiology stool profile includes comprehensive bacteriology and yeast cultures to identify beneficial flora, imbalanced flora including Clostridium species, and dysbiotic flora, as well as detection of infectious pathogens. Antimicrobial susceptibility testing to prescriptive and natural agents is also performed for appropriate bacterial and fungal species.

The Microbiology stool profile may be ideal for follow-up to the Comprehensive Stool Analysis when looking for a specific abnormality, to monitor certain microbial balances, or to assess treatment efficacy. We strongly recommend the Comprehensive Stool Analysis as the initial assessment for a more thorough evaluation of gut function.

The Microbiology Test is indicated for all chronic GI problems, for acute bowel pattern changes, and for many systemic diseases.

• Allergies

• Autoimmune Disorders

• Cardiovascular Disease

• Celiac Disease

• Diabetes

• Diarrhea, Constipation, Abdominal Distension

• Food Sensitivities

• Gastrointestinal Symptoms

• IBD

• IBS

• Immune System Imbalances

• Inflammation

• Joint Pain

• Leaky Gut

• Malnutrition

• Mood Disorders

• Neurological Disorders

• Weight Concerns

Sample Report - Microbiology Test

What Patients Might Benefit from the Food Sensitivities and Gut Imbalance Panel?

Symptoms and conditions associated with food sensitivities, nutritional deficiencies and other metabolic imbalances:

• ADHD

• Anxiety

• Asthma

• Autism Spectrum Disorders

• Autoimmune disease

• Behavioral Problems

• Celiac Disease

• Chronic Fatigue

• Crohn's Disease

• Dementia

• Depression

• Diabetes or insulin resistance

• Digestive Issues

• Fibromyalgia

• Headaches/Migraines

• Irritable Bowel Syndrome

• Lyme's Disease

• Metabolic Disorders

• Neurological Disorders

• Nutrient Deficiencies

• Obesity

• Skin Conditions (eczema, psoriasis, acne)

• Ulcerative Colitis

• Weight Gain

MosaicDX’s Food Sensitivities and Gut Imbalance Panel combines the Organic Acids Test (OAT) with the IgG Food MAP + Candida and Yeast providing comprehensive insights into your health. By identifying specific food sensitivities and understanding your metabolic profile, healthcare practitioners can tailor personalized interventions and dietary recommendations to your needs.

This powerful combination offers valuable insights into gut microbial balance and addresses potential irritants that can lead to poor nutrient absorption.

Sample Report - IgG Food MAP with Candida and Yeast

At-home collection. Meaningful insights. Personalized plan.

Thorne’s Gut Health Test provides a detailed analysis and a personalized plan that targets your GI discomfort to optimize wellness. Learn how to create meaningful changes in your gut’s microbiome with the combination of cutting-edge sequencing and patent-pending wipe technology that makes the sample collection process simple.

You should take this test if you

• Experience regular constipation or diarrhea

• Have excessive gas, bloating, abdominal pain, or nausea

• Have frequent heartburn

• Have IBS or IBD (or a family history of it)

• Have taken multiple courses of antibiotics

• Experience fatigue, low energy, or brain fog

Potential Indicators

The Gut Health Test identifies and quantifies bacteria, viruses, fungi, yeast, archaea, and parasites from a small stool sample that provides an unparalleled analysis of the composition of your microbiome.

The test can benefit individuals who are experiencing GI issues such as:

• IBS

• Diarrhea

• Constipation

• Gas

• Bloating

• Cramping

• Abdominal pain

• Nausea

• Heartburn

The test is useful for individuals who are looking to optimize gut health for:

• Athletic performance

• Recovery

• Immune function

• Biohacking

• Full-body health

• Mood/Brain Health

Why is Gut Health important?

The gut microbiome is the internal environment of the GI tract in which tens of trillions of microorganisms, including bacteria, fungi, archaea, and viruses all live together. And while the gut microbiome is influenced by demographic factors like age, genetics, and where a person lives, the gut microbiome’s composition is also greatly impacted by what comes into it at its entrance and what eventually exits it. Because the GI tract plays such a key role in multiple bodily functions, including digestion, vitamin synthesis, neurotransmitter support, and immune function, it’s important to keep this intricate system in balance to maintain the optimal function of these vital processes.

Thorne’s Gut Health Test takes a deep dive into your gut health and provides personalized recommendations for lifestyle, diet, and nutritional supplementation based on your unique gut biome composition.

NEW: A Novel Wipe for Easy Sample Collection

At Thorne, the cutting-edge technology we use has enabled us to introduce a new microbiome wipe that gently collects a stool sample with less fuss and mess. This one-of-a-kind wipe makes the collection process as simple as your regular bathroom routine. To improve the user experience of stool collection, the sample collection kit includes a dissolvable wipe in which the sample taken is dissolved in a DNA-stabilizing solution. The solution that contains the biological sample is sent to the sequencing lab in a sealable container.

How the measured microbes impact your health

Thorne’s Gut Health Test is sequenced using “whole genome shotgun sequencing,” which uses next-generation metagenomic sequencing that identifies and measures the DNA of every microorganism present in the stool sample, thus providing a detailed analysis of your gut’s health.

The test focuses on five foundational gut-health pillars that have been designated as such by the Institute for Functional Medicine:

Intestinal Permeability

Intestinal permeability is the measure of what types of material pass through your intestinal wall and how easily they do it. If the cells in the gut’s walls lose their “tight junctions,” then the gut becomes more permeable and allows more bacteria and toxins to pass from your gut into your bloodstream. The measure of your gut’s permeability will help you understand your risk for “leaky gut” based on the microorganisms present in your gut that can cause dysbiosis, or structural damage to the GI lining, or changes in regulation and signaling mechanisms.

Gut Dysbiosis

An optimal balance of the bacteria in your gut has a positive effect on your overall wellness. When the bacteria are not in balance, a condition known as dysbiosis, you can begin to experience undesirable GI symptoms. The Gut Health Test analyzes the balance of the good, bad, and conditional bacteria levels present in your gut and their research-backed associations to health, wellness, and disease conditions.

Digestion

Proper digestive health facilitates your body’s optimal absorption of nutrients. Age, prescription medications, over-the-counter products, surgery, and injuries can adversely impact your digestive capabilities. Learn whether your microbiome is populated with the right types of bacteria and in the right quantities to properly support the digestion of the food you eat. Poor digestion leads to undesirable symptoms like gas, bloating, diarrhea, and constipation.

Inflammation

An imbalanced gut biome can cause inflammation in the entire body. Understand your risk for inflammation and patterns of inflammatory activity based on the microorganisms that are present in your gut and their effects on whole-body inflammation.

Nervous System

An imbalanced gut biome can adversely affect your enteric nervous system – your “gut-brain axis” – which can adversely impact mental capacity, cognitive function, and mood. Learn how your bacterial makeup influences the real-time communication between your gut and your brain, which impacts your autonomic and central nervous systems, nutrient metabolism, sleep, mood, food cravings, stress levels, and more.

In addition to the five foundational gut pillars, the Gut Health Test measures these other key indicators of your gut’s health:

Pathogens

Your sample is screened for the bacteria, viruses, and parasites that can cause adverse GI symptoms or disease conditions.

Total Fungi

Learn if your gut has a normal level of fungi present or if you are at risk for developing a fungal overgrowth.

Short-Chain Fatty Acids

This measure analyzes the bacteria and genes that regulate your gut’s production of short-chain fatty acids (SCFAs). Your gut’s capability to produce the SCFAs is important for helping to maintain normal inflammation and immune function.

Diversity

Know the quantity and the quality of the various species that inhabit your gut. A lower beta diversity score indicates that the bacterial population of your gut is similar to others who have a healthy gut.

Probiotics

Review a quantitative analysis of your current probiotic levels and receive a list of commercially available probiotics to guide your supplementation choices or to help determine if your current probiotic supplementation is working for you.

Micronutrients

Learn your gut’s capability to produce B vitamins based on the abundance of B vitamin-producing bacteria present. B vitamins are essential for energy production, metabolism, blood cell production, immune function, and more.

Immune Readiness

Know your body’s readiness for an optimal immune response to foreign pathogens – bacteria, parasites, and viruses – based on your microbiome’s ecosystem.

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WHAT’S REPORTED

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Intestinal Permeability   •  Stool

Understand your personal risk for how easily particles can pass through your intestinal wall based on your microbiome composition.

Intestinal Permeability Score

Gut Dysbiosis   •  Stool

Measures the presence of any dysbiosis – an imbalance in the bacteria in the gut. An optimal balance of gut bacteria is associated with health, wellness, and a reduced risk of various disease conditions.

Good BacteriaBad BacteriaConditional BacteriaProbiotics

Digestion   •  Stool

Learn how your age, prescription medications, over-the-counter products, surgery, injuries, and various lifestyle factors can impact your digestive capabilities.

Digestion Score

Inflammation   •  Stool

Understand your personal risk for inflammation and patterns of inflammatory activity.

Inflammation Score

Enteric Nervous System Imbalance   •  Stool

Learn how your bacterial makeup impacts the real-time communication between your gut and brain and how it affects your autonomic and central nervous systems, nutrient metabolism, sleep, mood, food cravings, stress levels, and more.

Nervous System Imbalance Score

Diversity   •  Stool

The quality and the quantity of the microbes in your gut influence multiple metrics of health, weight, and metabolism, as well as your personal risk for disease. Your Beta-Diversity score compares your microbiome to the healthy microbiomes of others.

Beta-Diversity

Micronutrients   •  Stool

This is the score associated with your gut’s capability to produce folate (vitamin B9), vitamin B6, vitamin B12, and niacin (vitamin B3) based on the makeup of your microbiome.

NiacinB6FolateB12

Short-Chain Fatty Acids   •  Stool

Short-chain fatty acids (SCFA) help maintain a normal level of inflammation in the gut, while also supporting the gut’s immune function. Your score reflects the capability of your gut to produce SCFA based on your gut’s microbial composition.

ButyrateLactatePropionateValerate

Immune Readiness   •  Stool

Your gut’s microbial composition plays a key role in keeping you healthy when you are confronted with immune stressors. Your score is derived from an advanced proprietary equation.

Immune Score

Pathogens   •  Stool

Pathogens in the air, food, and drinking water can cause immediate and undesirable symptoms. Your sample is screened for 11 undesirable pathogens, including viruses and parasites.